P2X3 Recombinant Rabbit Monoclonal Antibody [PSH28-01]
cat.: HA724743
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH28-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse P2X3 aa 1-322. |
| Positive control: | Mouse dorsal root ganglion tissue lysate, Rat dorsal root ganglion tissue lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP |
1:5,000 1:500 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q3UR32 Mouse | P49654 Rat |
| Alternative names: | ATP receptor MGC129956 P2rx3 P2RX3_HUMAN P2X purinoceptor 3 P2X receptor subunit 3 P2X3 Purinergic receptor Purinergic receptor P2X ligand gated ion channel 3 Purinergic receptor P2X3 Purinoceptor P2X3 |
Images
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Fig1:
Western blot analysis of P2X3 on different lysates with Rabbit anti-P2X3 antibody (HA724743) at 1/5,000 dilution.
Lane 1: Mouse dorsal root ganglion tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat dorsal root ganglion tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 1 minute 40 seconds; ECL: K1802 Low expression: spleen (PMID: 15670583). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724743, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44 kDa Observed band size: 44-60 kDa |
|
Fig2:
Western blot analysis of P2X3 on Neuro-2a (Mouse brain neuroblastoma cell) cell lysate with Rabbit anti-P2X3 antibody (HA724743) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 40 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724743, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44 kDa Observed band size: 44-60 kDa |
|
Fig3:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Dorsal root ganglion Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724743, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Dorsal root ganglion Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724743, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Dorsal root ganglion Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724743, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spinal dorsal horn Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724743, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724743, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Low expression: spleen (PMID: 15670583). |
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Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Dorsal root ganglion Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724743, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig9:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Spinal dorsal horn Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724743, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig10:
Immunoprecipitation (IP)
P2X3 was immunoprecipitated in 0.2 mg Neuro-2a (Mouse brain neuroblastoma cell) cell lysate with HA724743 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724743 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Neuro-2a cell lysate (input) Lane 2: HA724743 IP in Neuro-2a cell lysate Lane 3: Rabbit IgG instead of HA724743 in Neuro-2a cell lysate Exposure time: 2 minutes 37 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724743, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 44 kDa Observed band size: 44-60 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.