ZO1 Recombinant Rabbit Monoclonal Antibody [PSH28-02]
cat.: HA724744
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-Fr, IF-Tissue, IF-Cell, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH28-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 195 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse ZO1 aa 1,401-1,745. |
| Positive control: | Mouse Kidney, Human Kidney, bEnd.3 (Mouse brain microvascular endothelial cell). |
| Subcellular location: | Cell membrane, Cell junction, tight junction, gap junction, Cell projection, podosome. |
| Recommended Dilutions:
IHC-Fr IF-Tissue IF-Cell FC(Intra) |
1:500 1:500 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q07157 Human | P39447 Mouse |
| Alternative names: | Tight junction protein 1 Tight junction protein ZO-1 Tight junction protein ZO1 TJP1 zo-1 Zo1 ZO1_HUMAN Zona occludens 1 Zona occludens 1 protein Zona occludens protein 1 Zonula occludens 1 protein Zonula occludens protein 1 |
Images
|
Fig1:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Kidney Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724744, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724744, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724744, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: bEnd.3 (Mouse brain microvascular endothelial cell) / RAW264.7 (Mouse monocytic macrophage leukemia cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724744, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Negative expression of ZO1 protein in RAW264.7 cell is consistent with the predicted expression pattern. |
|
Fig5:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) (Left, negative) / bEnd.3 (Mouse brain microvascular endothelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724744 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative expression of ZO1 protein in RAW264.7 cell is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.