CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-04]
cat.: HA724746
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH28-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CYP2E1 aa 251-493. |
| Positive control: | Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue FC(Intra) |
1:10,000 1:500-1:5,000 1:100 1:500 |
| Uniprot #: | SwissProt: P05181 Human | Q05421 Mouse | P05182 Rat |
| Alternative names: | 4-nitrophenol 2-hydroxylase CP2E1_HUMAN CPE1 CYP2E Cyp2e1 CYPIIE1 Cytochrome P450 2E1 Cytochrome P450 isozyme 3A Cytochrome P450 LM3A Cytochrome P450, family 2, subfamily e, polypeptide 1 cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 Cytochrome P450, subfamily IIE Cytochrome P450-ALC Cytochrome P450-J Cytochrome P450RLM6 EC 1.14.13.- EC 1.14.13.n7 Ethanol-inducible P450 Flavoprotein-linked monooxygenase MGC133529 Microsomal monooxygenase OTTHUMP00000020813 OTTHUMP00000046571 P450 ALC P450-J P450C2E P450J P450RLM6 Xenobiotic monooxygenase |
Images
|
Fig1:
Western blot analysis of CYP2E1 on different lysates with Rabbit anti-CYP2E1 antibody (HA724746) at 1/10,000 dilution.
Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Low expression: Spleen, colon (PMID: 15576447). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724746, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 57 kDa Observed band size: 50 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724746, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724746, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724746, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern. |
|
Fig5:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724746, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig6:
Application: Flow Cytometry (Intra)
Species: Human Sample: MCF7 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724746 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
|
Fig7:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: B16-F1 (Mouse melanoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724746 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.