Glucokinase Recombinant Rabbit Monoclonal Antibody [PSH28-07]
cat.: HA724749
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH28-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Glucokinase aa 1-465. |
| Positive control: | Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:2,500 1:5,000 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: P52792 Mouse | P17712 Rat |
| Alternative names: | ATP:D-hexose 6-phosphotransferase FGQTL3 GCK GK GLK Glucokinase Hexokinase D pancreatic isozyme Hexokinase type IV Hexokinase-4 Hexokinase-D HHF3 HK IV HK4 HKIV HXK4_HUMAN HXKP LGLK MODY2 |
Images
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Fig1:
Western blot analysis of Glucokinase on different lysates with Rabbit anti-Glucokinase antibody (HA724749) at 1/2,500 dilution.
Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Rat liver tissue lysate Lane 5: Rat kidney tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 8 seconds; ECL: K1801 Low expression: skeletal muscle (PMID: 23085254). Low expression of Glucokinase protein in kidney is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724749, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa |
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Fig2:
Western blot analysis of Glucokinase on different lysates with Rabbit anti-Glucokinase antibody (HA724749) at 1/2,500 dilution.
Lane 1: His-tagged Human Glucokinase recombinant protein Lane 2: His-tagged Human Hexokinase II recombinant protein Lysates/proteins at 10 ng/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724749, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Liver tissue from fasted mice. At low glucose concentrations, GCK binds to GCKR, and the resulting inactive complex is recruited into the hepatocyte nucleus (PMID: 24566088, PMID: 10713097). Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724749, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724749, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Low expression of Glucokinase protein in kidney is consistent with the predicted expression pattern. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Liver tissue from fasted rats. At low glucose concentrations, GCK binds to GCKR, and the resulting inactive complex is recruited into the hepatocyte nucleus (PMID: 24566088, PMID: 10713097). Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724749, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724749, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Low expression of Glucokinase protein in kidney is consistent with the predicted expression pattern. |
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Fig7:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Liver tissue from fasted mice. At low glucose concentrations, GCK binds to GCKR, and the resulting inactive complex is recruited into the hepatocyte nucleus (PMID: 24566088, PMID: 10713097). Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724749, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig8:
Application: Immunofluorescence (IF-tissue) Species: Rat Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Liver tissue from fasted rats. At low glucose concentrations, GCK binds to GCKR, and the resulting inactive complex is recruited into the hepatocyte nucleus (PMID: 24566088, PMID: 10713097). Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724749, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig9:
Immunoprecipitation (IP)
Glucokinase was immunoprecipitated in 0.2 mg Mouse liver tissue lysate with HA724749 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724749 at 1/2,500 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse liver tissue lysate (input) Lane 2: HA724749 IP in mouse tissue cell lysate Lane 3: Rabbit IgG instead of HA724749 in mouse liver tissue lysate Exposure time: 10 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724749, 1/2,500 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.