Biotin Conjugated ERK1/2 Recombinant Rabbit Monoclonal Antibody [SA43-03]
cat.: HA724765B
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: ELISA(Det), ELISA
Clonality: Monoclonal
Clone number: SA43-03
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within human ERK2 aa 180-379.
Positive control: Recombinant Human ERK1 (pT202+pY204)/ERK2 (pT185+pY187) protein (HA211528).
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  ELISA(Det)
  ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [SC58-01] to Phospho-ERK1 (T202+Y204)/ERK2 (T185+Y187) (Capture) (HA724764) and Recombinant Human ERK1 (pT202+pY204)/ERK2 (pT185+pY187) protein (HA211528) as the standard. The reference range value is 1,562-200,000 pg/mL.
Use at an assay dependent concentration.
Uniprot #: SwissProt: P27361 Human | P28482 Human | P63085 Mouse | Q63844 Mouse | P21708 Rat | P63086 Rat
Alternative names: ERK 1 ERK 2 ERK-2 ERK1 erk1/2 ERK2 ERT1 ERT2 Extracellular signal regulated kinase 1 Extracellular signal-regulated kinase 2 MAP kinase 1 MAP kinase 2 MAP kinase isoform p42 MAP kinase isoform p44 MAPK 1 MAPK 2 MAPK 3 Mapk1 MAPK2 MAPK3 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 2 MK01_HUMAN p38 p40 p41 p42-MAPK PRKM 2
Images
HA724765B_1.png Fig1: Sandwich ELISA analysis of Human ERK1 (pT202+pY204)/ERK2 (pT185+pY187) protein matched pair antibodies

Capture: HA724764, Phospho-ERK1 (T202+Y204)/ERK2 (T185+Y187) Rabbit mAb [SC58-01]
Detector: HA724765, ERK1/2 Rabbit mAb [SA43-03]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724764) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ERK1 (pT202+pY204)/ERK2 (pT185+pY187) protein (HA211528) starting from 200,000 pg/mL to 0 pg/mL and detect antibody (HA724765, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724765B_2.png Fig2: Interpolated concentrations of native ERK1 (pT202+pY204)/ERK2 (pT185+pY187) in NIH/3T3 stimulated or unstimulated with 200nM PMA for 30 minutes, NIH/3T3 cell extract samples based on a 1,000 µg/mL extract load.

Capture: HA724764, Human Phospho-ERK1 (T202+Y204)/ERK2 (T185+Y187) Rabbit mAb [SC58-01]
Detector: HA724765, ERK1/2 Rabbit mAb [SA43-03]

The concentrations of ERK1 (pT202+pY204)/ERK2 (pT185+pY187) were measured in duplicates, interpolated from the ERK1 (pT202+pY204)/ERK2 (pT185+pY187) standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ERK1 (pT202+pY204)/ERK2 (pT185+pY187) concentration was determined to be 116 ng/mL in stimulated NIH/3T3 cell extract and 12 ng/mL in unstimulated NIH/3T3 cell extract.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.