STIM2 Recombinant Rabbit Monoclonal Antibody [PSH28-30]
cat.: HA724771
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH28-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human STIM2 aa 401-746. |
| Positive control: | HEK-293 (Human embryonic kidney cell) cell lysate, BT-20 (Human breast cancer cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB FC(Intra) |
1:2,000 1:200 |
| Uniprot #: | SwissProt: Q9P246 Human | P83093 Mouse Entrez Gene: 117087 Rat |
| Alternative names: | FLJ39527 KIAA1482 STIM 2 STIM2 STIM2_HUMAN Stromal interaction molecule 2 |
Images
|
Fig1:
Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (HA724771) at 1/2,000 dilution.
Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate Lane 2: BT-20 (Human breast cancer cell) cell lysate Lane 3: MDA-MB-231 (Human breast cancer cell) cell lysate Lane 4: MCF7 (Human breast cancer cell) cell lysate Lane 5: T-47D (Human breast ductal carcinoma cell) cell lysate Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 7: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Low expression: T-47D (PMID: 31464639) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724771, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 84 kDa Observed band size: 100 kDa |
|
Fig2:
Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (HA724771) at 1/2,000 dilution.
Lane 1: Mouse spleen tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat spleen tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724771, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 84 kDa Observed band size: 100 kDa |
|
Fig3:
Application: Flow Cytometry (Intra)
Species: Human Sample: HEK-293 (Human embryonic kidney cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724771 (1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.