Jagged1 Recombinant Rabbit Monoclonal Antibody [PSH28-36]
cat.: HA724777
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH28-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 134 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Jagged1 aa 1,057-1,218. |
| Positive control: | Hep G2 (Human hepatocellular carcinoma cell) cell lysate, NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, Hepa1-6 (Mouse liver cancer cell) cell lysate, bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P78504 Human | Q9QXX0 Mouse |
| Alternative names: | AGS AHD AWS CD 339 CD339 CD339 antigen Headturner hJ1 Htu Jag 1 Jag1 JAG1_HUMAN Jagged 1 Jagged1 (Alagille syndrome) Jagged1 JAGL1 MGC104644 OTTHUMP00000030278 Protein jagged-1 Ser 1 Ser1 Serrate 1 Slalom |
Images
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Fig1:
Western blot analysis of Jagged1 on different lysates with Rabbit anti-Jagged1 antibody (HA724777) at 1/2,000 dilution.
Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate Lane 3: LNCaP (Human prostate cancer cell) cell lysate Lane 4: HUVEC (Human umbilical vein endothelial cell) cell lysate Lane 5: MOLT-4 (Human acute T-Cell leukemia cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 MOLT-4 is a negative control (PMID: 30231940). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724777, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 134 kDa Observed band size: 150-180 kDa |
|
Fig2:
Western blot analysis of Jagged1 on different lysates with Rabbit anti-Jagged1 antibody (HA724777) at 1/5,000 dilution.
Lane 1: Hepa1-6 (Mouse liver cancer cell) cell lysate Lane 2: bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724777, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 134 kDa Observed band size: 150-180 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.