PEDF Recombinant Rabbit Monoclonal Antibody [PSH28-40]
cat.: HA724781
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Pig |
| Applications: | WB, FC(Intra), IP |
| Clonality: | Monoclonal |
| Clone number: | PSH28-40 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PEDF aa 1-418. |
| Positive control: | K-562 (Human chronic myelogenous leukemia cell) cell lysate, Y79 (Human retinoblastoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysates, NIH/3T3 (Mouse fibroblast) cell lysate, 3T3-L1 (Mouse embryonic fibroblast) cell lysate, C2C12 (Mouse myoblast) cell lysate, NRK (Rat kidney cell) cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Mouse stomach tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Human serum lysate, Mouse plasma lysate, Rat plasma lysate, Pig liver tissue lysate, Pig brain tissue lysate. |
| Subcellular location: | Secreted, Melanosome. |
| Recommended Dilutions:
WB FC(Intra) IP |
1:2,500 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: P36955 Human | P97298 Mouse Entrez Gene: 287526 Rat |
| Alternative names: | Cell proliferation-inducing gene 35 protein EPC 1 EPC-1 EPC1 OI12 OI6 PEDF PEDF_HUMAN PIG 35 PIG35 Pigment epithelium derived factor Pigment epithelium-derived factor Proliferation inducing protein 35 Serine (or cysteine) proteinase inhibitor serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 Serpin F1 Serpin family F member 1 Serpin peptidase inhibitor Serpin peptidase inhibitor clade F member 1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 SERPINF 1 Serpinf1 |
Images
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Fig1:
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: Y79 (Human retinoblastoma cell) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes 6 seconds; ECL: K1801 MCF7 is a negative control (PMID: 16740777). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 50 kDa |
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Fig2:
Western blot analysis of PEDF on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 50 kDa |
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Fig3:
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate Lane 2: 3T3-L1 (Mouse embryonic fibroblast) cell lysate Lane 3: C2C12 (Mouse myoblast) cell lysate Lane 4: NRK (Rat kidney cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 18 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 50 kDa |
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Fig4:
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: Mouse liver tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse stomach tissue lysate Lane 4: Rat liver tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 50 kDa |
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Fig5:
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: Human serum lysate Lane 2: Mouse plasma lysate Lane 3: Rat plasma lysate Lysates/proteins at 10 µg/Lane. Exposure time: 18 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 50 kDa |
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Fig6:
Western blot analysis of PEDF on different lysates with Rabbit anti-PEDF antibody (HA724781) at 1/2,500 dilution.
Lane 1: Pig liver tissue lysate Lane 2: Pig brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 50 kDa |
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Fig7:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724781 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig8:
Immunoprecipitation (IP)
PEDF was immunoprecipitated in 0.2 mg Hep G2 (Human hepatocellular carcinoma cell) cell lysate with HA724781 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724781 at 1/2,500 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Hep G2 cell lysate (input) Lane 2: HA724781 IP in Hep G2 cell lysate Lane 3: Rabbit IgG instead of HA724781 in Hep G2 cell lysate Exposure time: 12 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724781, 1/2,500 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 46 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.