RUVBL2 Recombinant Antibody [A1E12-R] - Rabbit IgG (Chimeric)
cat.: HA724802
Product Type: Recombinant Chimeric, primary antibodies
Species reactivity: Human, Mouse, Rat, Green monkey
Applications: WB, IF-Cell, IHC-P, FC(Intra)
Clone number: A1E12-R
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Immunogen: Recombinant protein within Human RUVB2 aa 130-321 / 463.
Positive control: HeLa (Human cervical adenocarcinoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Subcellular location: Nucleus matrix, nucleoplasm, cytoplasm, membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC(Intra)
1:2,000
1:500
1:2,000
1:1,000
Uniprot #: SwissProt: Q9Y230 Human | Q9WTM5 Mouse
Entrez Gene: 292907 Rat
Alternative names: 48 kDa TATA box-binding protein-interacting protein 48 kDa TBP-interacting protein 48-kDa TATA box-binding protein-interacting protein 48-kDa TBP-interacting protein 51 kDa erythrocyte cytosolic protein CGI-46 EC=3.6.1.- ECP-51 ECP51 Erythrocyte cytosolic protein, 51-KD INO80 complex subunit J INO80J MGC144733 MGC144734 MGC52995 mp47 p47 p47 protein Repressing pontin 52 Reptin 52 REPTIN RuvB (E coli homolog)-like 2 RUVB, E. coli, homolog-like 2 RuvB-like 2 (E. coli) RuvB-like 2 RuvB-like protein 2 RUVB2 RUVB2_HUMAN RUVBL2 RVB2 TAP54-beta TATA box-binding protein-interacting protein, 48-KD TBP-interacting protein, 48-KD TIH2 TIP48 TIP49b TIP60-associated protein 54-beta wu:fi25f01 zreptin
Images
HA724802_1.jpg Fig1: Western blot analysis of RUVBL2 on different lysates with Rabbit anti-RUVBL2 antibody (HA724802) at 1/2,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane)
Lane 2: 293T (Human embryonic kidney cell) cell lysate (15 µg/Lane)
Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate (15 µg/Lane)
Lane 4: C6 (Rat glioma cell) cell lysate (15 µg/Lane)
Lane 5: COS-1 (African green monkey kidney fibroblast) cell lysate (15 µg/Lane)
Lane 6: Mouse testis tissue lysate (30 µg/Lane)
Lane 7: Rat testis tissue lysate (30 µg/Lane)

Exposure time: 6 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724802, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 51 kDa
Observed band size: 51 kDa
HA724802_2.jpg Fig2: Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA724802, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
HA724802_3.jpg Fig3: Application: Immunocytochemistry (IF-cell)

Species: Rat
Sample: C6 (Rat glioma cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA724802, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
HA724802_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Testis
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724802_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Testis
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724802_6.jpg Fig6: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Testis
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
HA724802_7.jpg Fig7: Application: Flow Cytometry (Intra)

Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.

Primary antibody: HA724802 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.