TGFBI Recombinant Rabbit Monoclonal Antibody [PSH28-78]
cat.: HA724809
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH28-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TGFBI aa 1-683. |
| Positive control: | A549 (Human lung adenocarcinoma cell) cell lysate, A549 treated with 5ng/mL hTGFβ1 for 48 hours add 300ng/mL BFA for last 16 hours cell lysate, NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysates. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:200 1:2,000-1:10,000 |
| Uniprot #: | SwissProt: Q15582 Human |
| Alternative names: | RGD containing collagen associated protein AI181842 AI747162 Beta ig Beta ig h3 Beta ig-h3 BGH3_HUMAN Big h3 BIGH3 CDB1 CDG2 CDGG1 CSD CSD1 CSD2 CSD3 EBMD Kerato epithelin Kerato-epithelin LCD1 MGC150270 RGD CAP RGD-CAP RGD-containing collagen-associated protein TGFBI TGFBI transforming growth factor, beta induced, 68kDa Transforming growth factor beta induced protein ig h3 Transforming growth factor-beta-induced protein ig-h3 |
Images
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Fig1:
Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (HA724809) at 1/2,000 dilution.
Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate Lane 2: A549 treated with 5ng/mL hTGFβ1 for 48 hours add 300ng/mL BFA for last 16 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724809, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 75 kDa Observed band size: 70 kDa |
|
Fig2:
Western blot analysis of TGFBI on NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate with Rabbit anti-TGFBI antibody (HA724809) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724809, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 75 kDa Observed band size: 70 kDa |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: A549 (Human lung adenocarcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724809, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Squamous cell carcinoma of the lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724809, 1/8,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724809, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724809, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.