CD44 Recombinant Rabbit Monoclonal Antibody [PSH28-99]
cat.: HA724828
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH28-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD44 aa 1-250. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, T98G (Human brain glioblastoma cell) cell lysate. |
| Subcellular location: | Cell membrane, Cell projection, microvillus, Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: P16070 Human |
| Alternative names: | LHR BA-1 CD 44 CD44 CD44 antigen CD44 molecule (Indian blood group) CD44 molecule CD44_HUMAN CDw44 CDW44 antigen Cell surface glycoprotein CD44 chondroitin sulfate proteoglycan 8 CSPG8 ECMR-III Epican Extracellular matrix receptor III GP90 lymphocyte homing/adhesion receptor HCELL hematopoietic cell E- and L-selectin ligand Heparan sulfate proteoglycan Hermes antigen homing function and Indian blood group system HSA HUTCH-I HUTCH1 HUTCHI Hyaluronate receptor IN INLU-related p80 Glycoprotein MC56 MDU2 MDU3 MGC10468 MIC4 MUTCH I MUTCH1 PGP-1 PGP-I PGP1 Phagocytic glycoprotein 1 Phagocytic glycoprotein I Soluble CD44 |
Images
|
Fig1:
Western blot analysis of CD44 on different lysates with Rabbit anti-CD44 antibody (HA724828) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate Lane 3: LNCaP (Human prostate cancer cell) cell lysate Lane 4: A549 (Human lung adenocarcinoma cell) cell lysate Lane 5: MDA-MB-231 (Human breast cancer cell) cell lysate Lane 6: HUVEC (Human umbilical vein endothelial cell) cell lysate Lane 7: T98G (Human brain glioblastoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 7 seconds; ECL: K1801 The staining pattern is consistent with reference (PMID: 19507218, PMID: 20670691). Negative control: LNCaP (PMID: 31331331) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724828, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 82 kDa Observed band size: 82 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) / LNCaP (Human prostate cancer cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724828, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Negative control: LNCaP (PMID: 31331331). |
|
Fig3:
Application: Flow Cytometry Species: Human Sample: LNCaP (Human prostate cancer cell) (Left, Negative) / PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724828 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: LNCaP (PMID: 31331331). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.