Human G-CSF Recombinant Rabbit Monoclonal Antibody [PSH13-12]
cat.: HA725001
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human G-CSF protein aa 31-204 (HA210840). |
| Positive control: | Recombinant Human G-CSF protein (HA210840). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-13] to Human G-CSF antibody (Detector) (HA725002) and recombinant Human G-CSF protein (HA210840) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P09919 Human |
| Alternative names: | C17orf33 Colony stimulating factor 3 (granulocyte) Colony stimulating factor 3 CSF 3 CSF beta CSF3 CSF3_HUMAN CSF3OS Csfg Filgrastim G-CSF GCSA GCSF Granulocyte colony stimulating factor Granulocyte colony-stimulating factor Lenograstim Macrophage granulocyte inducer 2 MGC45931 MGI 2 Pluripoietin |
Images
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Fig1:
Sandwich ELISA analysis of Human G-CSF matched pair antibodies Capture: HA725001, Human G-CSF Rabbit mAb [PSH13-12] Detector: HA725002, Human G-CSF Rabbit mAb [PSH13-13] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725001) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/mlovernight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human G-CSF protein (HA210840) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA725002, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of spiked G-CSF in cell culture media samples. Capture: HA725001, Human G-CSF Rabbit mAb [PSH13-12] Detector: HA725002, Human G-CSF Rabbit mAb [PSH13-13] The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.