Human CXCL10 / IP-10 Recombinant Rabbit Monoclonal Antibody [PSH12-37]
cat.: HA725008
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CXCL10 aa 22-98 (HA210896). |
| Positive control: | Recombinant Human CXCL10 protein (HA210896). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-36] to Human CXCL10 / IP-10 antibody (Capture) (HA725007) and Recombinant Human CXCL10 / IP-10 protein (HA210896) as the standard. The reference range value is 1.6-200 pg/ml. |
| Uniprot #: | SwissProt: P02778 Human |
| Alternative names: | Interferon gamma induced factor MOB1, mouse, homolog of Interferon gamma induced protein 10 10 kDa interferon gamma induced protein 10 kDa interferon gamma-induced protein C X C motif chemokine 10 C7 Chemokine (C X C motif) ligand 10 Chemokine CXC motif ligand 10 Crg 2 CRG2 CXCL10 CXCL10(1-73) CXL10_HUMAN Gamma IP10 Gamma-IP10 gIP 10 GIP10 IFI10 INP 10 INP10 Interferon activated gene 10 Interferon activated gene 10 Interferon gamma induced cell line Interferon inducible cytokine IP 10 Interferon inducible cytokine IP10 IP 10 IP-10 Mob 1 MOB1 Protein 10 from interferon (gamma) induced cell line SCYB10 Small inducible cytokine B10 Small inducible cytokine B10 precursor Small inducible cytokine subfamily B (Cys X Cys) member 10 Small inducible cytokine subfamily B CXC member 10 Small inducible cytokine subfamily B, member 10 Small-inducible cytokine B10 |
Images
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Fig1:
Sandwich ELISA analysis of human CXCL10 matched pair antibodies Capture: HA725007, Human CXCL10 / IP-10 Rabbit mAb [PSH12-36] Detector: HA725008, Human CXCL10 / IP-10 Rabbit mAb [PSH12-37] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725007) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL10 protein (HA210896) starting from 200 pg/ml to 0 pg/ml and detect antibody (HA725008, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL10 in THP-1 supernatant samples. Capture: HA725007, Human CXCL10 / IP-10 Rabbit mAb [PSH12-36] Detector: HA725008, Human CXCL10 / IP-10 Rabbit mAb [PSH12-37] Interpolated concentration of native CXCL10 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL10 concentration was determined to be 2993 pg/mL in THP-1+hu IFN-γ (1μg/ml 8h) then LPS (1μg/mL overnight) sups (+) sups, 7 pg/ml in THP-1+hu IFN-γ (1μg/ml 8h) then LPS (1μg/mL overnight) sups (-) cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.