Human CCL20/MIP-3 alpha Recombinant Rabbit Monoclonal Antibody [PSH12-33]
cat.: HA725011
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL20/MIP-3 alpha aa 27-96 (HA211012). |
| Positive control: | Recombinant Human CCL20/MIP-3 alpha protein (HA211012). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-32] to Human CCL20/MIP-3 alpha antibody (Capture) (HA725010) and Recombinant Human CCL20/MIP-3 alpha protein (HA211012) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P78556 Human |
| Alternative names: | Beta chemokine exodus 1 Beta-chemokine exodus-1 C C motif chemokine ligand 20 C-C motif chemokine 20 CC chemokine LARC Ccl20 CCL20(2-70) CCL20_HUMAN Chemokine (C C motif) ligand 20 Chemokine C-C motif chemokine 20 Chemokine CC motif ligand 20 CKb4 Exodus 1 Exodus LARC Liver and activation regulated chemokine Liver and activation-regulated chemokine Macrophage inflammatory protein 3 alpha MIP 3 alpha MIP 3A MIP-3-alpha MIP-3a MIP3 alpha MIP3A SCYA20 Small inducible cytokine A20 Small inducible cytokine subfamily A (Cys Cys) member 20 Small-inducible cytokine A20 ST38 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL20/MIP-3 alpha matched pair antibodies Capture: HA725010, Human CCL20/MIP-3 alpha Rabbit mAb [PSH12-32] Detector: HA725011, Human CCL20/MIP-3 alpha Rabbit mAb [PSH12-33] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725010) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL20/MIP-3 alpha protein (HA211012) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA725011, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL20 in THP-1 supernatant treated or untreated with Hu-IFN-r and LPS for overnight. Capture: HA725010, Human CCL20/MIP-3 alpha Rabbit mAb [PSH12-32] Detector: HA725011, Human CCL20/MIP-3 alpha Rabbit mAb [PSH12-33] Interpolated concentration of native CCL20 was measured in duplicate at different sample concentrations and interpolated from the CCL20 standard curves. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL20 concentration was determined to be 2,126 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.