Human IL-13 Recombinant Rabbit Monoclonal Antibody [PSH12-38]
cat.: HA725013
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-13 aa 25-146 (HA210908). |
| Positive control: | Recombinant Human IL-13 protein (HA210908). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-39] to Human IL-13 antibody (Detector) (HA725014) and Recombinant Human IL-13 protein (HA210908) as the standard. The reference range value is 31.3-4,000 pg/mL. |
| Uniprot #: | SwissProt: P35225 Human |
| Alternative names: | Allergic rhinitis ALRH BHR 1 BHR1 Bronchial hyperresponsiveness 1 (bronchial asthma) IL 13 IL-13 Il13 IL13_HUMAN Interleukin 13 Interleukin-13 interleukin13 MGC116786 MGC116788 MGC116789 NC 30 NC30 P 600 P600 |
Images
|
Fig1:
Sandwich ELISA analysis of human IL-13 matched pair antibodies Capture: HA725013, Human IL-13 Rabbit mAb [PSH12-38] Detector: HA725014, Human IL-13 Rabbit mAb [PSH12-39] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725013) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-13 protein (HA210908) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725014, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL-13 in human PBMC cell culture supernatant. Capture: HA725013, Human IL-13 Rabbit mAb [PSH12-38] Detector: HA725014, Human IL-13 Rabbit mAb [PSH12-39] PBMC cells were stimulated with 10 µg/ml PHA-M and incubated for 6 days. The concentrations of IL-13 were interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated 50%. The mean IL-13 concentration was determined to be 5,701 pg/ml in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.