Human IL-25 Recombinant Rabbit Monoclonal Antibody [PSH15-24]
cat.: HA725017
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH15-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-25 aa 33-177 (HA211098). |
| Positive control: | Recombinant Human IL-25 protein (HA211098). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-23] to Human IL-25 antibody (Capture) (HA725016) and Recombinant Human IL-25 protein (HA211098) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: Q9H293 Human |
| Alternative names: | IL 17E IL 25 IL-17E IL-25 IL17e IL25 IL25_HUMAN Interleukin 17E Interleukin 25 Interleukin-17E Interleukin-25 Interleukin17E Interleukin25 OTTHUMP00000027946 OTTHUMP00000246238 UNQ3120/PRO10272 |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-25 matched pair antibodies Capture: HA725016, Human IL-25 Rabbit mAb [PSH15-23] Detector: HA725017, Human IL-25 Rabbit mAb [PSH15-24] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725016) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-25 protein (HA211098) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725017, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.