Mouse GM-CSF Recombinant Rabbit Monoclonal Antibody [PSH12-41]
cat.: HA725020
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse GM-CSF aa 18-141 (HA210930). |
| Positive control: | Recombinant Mouse GM-CSF protein (HA210930). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-40] to Mouse GM-CSF antibody (Capture) (HA725019) and Recombinant Mouse GM-CSF protein (HA210930) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P01587 Mouse |
| Alternative names: | Colony stimulating factor 2 (granulocyte-macrophage) Colony Stimulating Factor 2 Colony stimulating factor Colony-stimulating factor CSF 2 CSF CSF2 CSF2_HUMAN GM-CSF GMCSF Granulocyte Macrophage Colony Stimulating Factor Granulocyte-macrophage colony-stimulating factor MGC131935 MGC138897 MGI1GM Molgramostin Pluripoietin-a Sargramostim |
Images
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Fig1:
Sandwich ELISA analysis of 0 Mouse GM-CSF matched pair antibodies Capture: HA725019, Mouse GM-CSF Rabbit mAb [PSH12-40] Detector: HA725020, Mouse GM-CSF Rabbit mAb [PSH12-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725019) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GM-CSF protein (HA210930) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA725020, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native GM-CSF in RAW264.7 supernatant treated or untreated with LPS for 48h. Capture: HA725019, Mouse GM-CSF Rabbit mAb [PSH12-40] Detector: HA725020, Mouse GM-CSF Rabbit mAb [PSH12-41] Interpolated concentration of native GM-CSF was measured in duplicate at different sample concentrations and interpolated from the GM-CSF standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 17,161 pg/mL in neat RAW264.7 treated supernatant and 126 pg/mL in untreated RAW264.7 supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.