Mouse/Rat CXCL1/GRO alpha Recombinant Rabbit Monoclonal Antibody [PSH13-14]
cat.: HA725028
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CXCL1 aa 25-96 (HA210792). |
| Positive control: | Recombinant Mouse CXCL1 protein (HA210792) or Rat CXCL1 protein (HA211010). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-15] to Mouse/Rat CXCL1/GRO alpha antibody (Detector) (HA725029) and recombinant Mouse CXCL1/GRO protein (HA210792) or recombinant Rat CXCL1 protein (HA211010) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: P12850 Mouse | P14095 Rat |
| Alternative names: | C-X-C motif chemokine 1 Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) chemokine (C-X-C motif) ligand 1 CINC-1 CXCL1 Cytokine-induced neutrophil chemoattractant 1 Fibroblast secretory protein Fsp Gro 1 Gro A Gro GRO protein, alpha GRO-alpha(1-73) GRO-alpha(6-73) Gro1 GRO1 oncogene (melanoma growth stimulating activity, alpha) GRO1 oncogene (melanoma growth-stimulating activity) Gro1 oncogene GROa GROA_HUMAN Growth-regulated alpha protein KC KC chemokine, mouse, homolog of melanoma growth stimulatory activity alpha Melanoma growth stimulatory activity Melanoma growth stimulatory activity, alpha MGSA alpha MGSA MGSA-a N51 NAP-3 NAP3 Neutrophil-activating protein 3 Platelet-derived growth factor-inducible protein KC Scyb 1 Scyb1 Secretory protein N51 Small inducible cytokine subfamily B, member 1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CXCL1 matched pair antibodies Capture: HA725028, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14] Detector: HA725029, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725028) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CXCL1 protein (HA210792) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA725029, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Rat CXCL1 matched pair antibodies Capture: HA725028, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14] Detector: HA725029, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725028) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat CXCL1 protein (HA211010) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA725029, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of native CXCL1 in mouse samples. Capture: HA725028, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14] Detector: HA725029, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15] Interpolated concentration of native CXCL1 was measured in duplicate at different sample concentrations and interpolated from the CXCL1 standard curves. Undiluted samples was100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 266 pg/mL in NIH/3T3. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.