Mouse/Rat IGF1 Recombinant Rabbit Monoclonal Antibody [PSH12-42]
cat.: HA725037
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IGF1 aa 49-118 (HA210936). |
| Positive control: | Recombinant Mouse IGF1 protein (HA210936). Recombinant Rat IGF1 protein (HA211009). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-43] to Mouse/Rat IGF1 antibody (Detector) (HA725038) and Recombinant Mouse/Rat IGF1 protein (HA210936) as the standard. The reference range value is 781-50,000 pg/mL. |
| Uniprot #: | SwissProt: P05017 Mouse | P08025 Rat |
| Alternative names: | IBP1 IGF I IGF IA IGF IB IGF-I Igf1 IGF1_HUMAN IGF1A IGFI IGFIA Insulin like growth factor 1 (somatomedin C) Insulin like growth factor 1 Insulin like growth factor IA Insulin like growth factor IB Insulin-like growth factor I Mechano growth factor MGF OTTHUMP00000195080 OTTHUMP00000195081 OTTHUMP00000195082 OTTHUMP00000195083 OTTHUMP00000195084 Somatomedia C Somatomedin C Somatomedin-C |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA210936) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Rat IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA211009) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of native Mouse IGF1 in mouse samples. Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Interpolated concentration of native IGF1 was measured in duplicate at different sample concentrations. Undiluted samples were 1.25% mouse serum. The mean IGF1 concentration was determined to be 1,584,637 pg/mL in mouse serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.