Biotin Conjugated Mouse CCL2 / MCP-1 Recombinant Rabbit Monoclonal Antibody [PSH12-45]
cat.: HA725042B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-45 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CCL2 aa 24-148 (HA210775). |
| Positive control: | Recombinant Mouse CCL2 protein (HA210775). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-44] to Mouse CCL2 antibody (Capture) (HA725040) and Recombinant Mouse CCL2 protein (HA210775) as the standard. The reference range value is 15.6-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10148 Mouse |
| Alternative names: | C-C motif chemokine 2 CCL2 CCL2_HUMAN Chemokine (C C motif) ligand 2 GDCF 2 GDCF-2 GDCF2 HC11 HSMCR30 JE MCAF MCP 1 MCP-1 MCP1 MGC9434 Monocyte chemoattractant protein 1 Monocyte chemotactic and activating factor Monocyte chemotactic protein 1 Monocyte chemotactic protein-1 Monocyte secretory protein JE SCYA2 Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) Small inducible cytokine A2 Small inducible cytokine subfamily A (Cys Cys), member 2 Small-inducible cytokine A2 SMC CF SMC-CF SMCCF |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CCL2 matched pair antibodies Capture: HA725040, Mouse CCL2 Rabbit mAb [PSH12-44] Detector: HA725041, Mouse CCL2 Rabbit mAb [PSH12-45] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725040) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CCL2 protein (HA210775) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725041, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL2 in J774A.1 cell culture supernatant. Capture: HA725040, Mouse CCL2 Rabbit mAb [PSH12-44] Detector: HA725041, Mouse CCL2 Rabbit mAb [PSH12-45] J774A.1 were stimulated with 20 ng/ml IFN-γ and 1 μg/ml LPS incubated for 1 day. The concentrations of CCL2 were measured in duplicate and interpolated from the CCL2 standard curves. Undiluted samples are IFN-γ and LPS stimulated J774A.1 cell culture supernatant 1.56% and unstimulated J774A.1 cell culture supernatant 50%. The mean CCL2 concentration was determined to be 13,358 pg/ml in IFN-γ and LPS stimulated J774A.1 cell culture supernatant and 1,679 pg/ml in the unstimulated J774A.1 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.