Rat CRP Recombinant Rabbit Monoclonal Antibody [PSH12-79]
cat.: HA725049
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat CRP aa 20-230 (HA211008). |
| Positive control: | Recombinant Rat CRP protein (HA211008). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-80] to Rat CRP antibody (Detector) (HA725050) and Recombinant Rat CRP protein (HA211008) as the standard. The reference range value is 78.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P48199 Rat |
| Alternative names: | Pentraxin 1, short C reactive protein C reactive protein pentraxin related C-reactive protein(1-205) CRP CRP_HUMAN MGC88244 Pentraxin 1 PTX 1 PTX1 |
Images
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Fig1:
Sandwich ELISA analysis of Rat CRP matched pair antibodies Capture: HA725049, Rat CRP Rabbit mAb [PSH12-79] Detector: HA725050, Rat CRP Rabbit mAb [PSH12-80] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725049) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat CRP protein (HA211008) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725050, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CRP in rat serum samples. Capture: HA725049, Rat CRP Rabbit mAb [PSH12-79] Detector: HA725050, Rat CRP Rabbit mAb [PSH12-80] The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curve and corrected for sample dilution. Undiluted samples are rat serum 0.0031%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 294 µg/ml in rat serum. |
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Fig3:
Interpolated concentrations of native CRP in rat heart extract samples based on a 1,000 µg/ml extract load. Capture: HA725049, Rat CRP Rabbit mAb [PSH12-79] Detector: HA725050, Rat CRP Rabbit mAb [PSH12-80] The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curve and corrected for sample dilution. Undiluted samples are rat heart extract 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 230 ng/ml in rat heart extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.