HRP Conjugated Rat CRP Recombinant Rabbit Monoclonal Antibody [PSH12-80]
cat.: HA725051H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat CRP aa 20-230 (HA211008). |
| Positive control: | Recombinant Rat CRP protein (HA211008). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-79] to Rat CRP antibody (Capture) (HA725049) and Recombinant Rat CRP protein (HA211008) as the standard. The reference range value is 140-33,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P48199 Rat |
| Alternative names: | Pentraxin 1, short C reactive protein C reactive protein pentraxin related C-reactive protein(1-205) CRP CRP_HUMAN MGC88244 Pentraxin 1 PTX 1 PTX1 |
Images
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Fig1:
Sandwich ELISA analysis of Rat CRP matched pair antibodies Capture: HA725049, Rat CRP Rabbit mAb [PSH12-79] Detector: HA725050, Rat CRP Rabbit mAb [PSH12-80] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725049) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat CRP protein (HA211008) starting from 33,000 pg/ml to 0 pg/ml and detect antibody (HA725050, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.