Biotin Conjugated Human CCL5 Recombinant Rabbit Monoclonal Antibody [PSH13-17]
cat.: HA725057B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH13-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL5 aa 24-91 (HA210998). |
| Positive control: | Recombinant Human CCL5 protein (HA210998). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-16] to Human CCL5 antibody (Capture) (HA725055) and recombinant Human CCL5 protein (HA210998) as the standard. The reference range value is 7.8-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P13501 Human |
| Alternative names: | Beta chemokine RANTES Beta chemokine RANTES precursor C C motif chemokine 5 CCL 5 CCL5 CCL5_HUMAN Chemokine (C C motif) ligand 5 Chemokine CC Motif Ligand 5 D17S136E EoCP Eosinophil chemotactic cytokine MGC17164 RANTES(4-68) Regulated upon activation normally T expressed and presumably secreted SCYA 5 SCYA5 SIS delta SIS-delta SISd Small inducible cytokine A5 (RANTES) Small inducible cytokine A5 Small inducible cytokine subfamily A (Cys Cys) member 5 Small-inducible cytokine A5 T cell specific protein p288 T cell specific protein RANTES T cell specific RANTES protein T cell-specific protein P228 T-cell-specific protein RANTES TCP 228 TCP228 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL5 matched pair antibodies Capture: HA725055, Human CCL5 Rabbit mAb [PSH13-16] Detector: HA725056, Human CCL5 Rabbit mAb [PSH13-17] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725055) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL5 protein (HA210998) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725056, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL5 in human samples. Capture: HA725055, Human CCL5 Rabbit mAb [PSH13-16] Detector: HA725056, Human CCL5 Rabbit mAb [PSH13-17] Interpolated concentration of native CCL5 was measured in duplicate at different sample concentrations and interpolated from the CCL5 standard curves. Undiluted samples were 2% human serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL5 concentration was determined to be 11,647 pg/mL in NK-92 and 182 pg/ml in Raji cell supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.