Human Leptin Receptor Recombinant Rabbit Monoclonal Antibody [PSH14-71]
cat.: HA725058
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH14-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Leptin Receptor aa 22-839 (HA211148) |
| Positive control: | Recombinant Human Leptin Receptor protein (HA211148). |
| Subcellular location: | Cell membrane, Basolateral cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-72] to Human Leptin Receptor antibody (Detector) (HA725059) and Recombinant Human Leptin Receptor protein (HA211148) as the standard. The reference range value is 156-20,000 pg/mL. |
| Uniprot #: | SwissProt: P48357 Human |
| Alternative names: | CD 295 CD295 CD295 antigen Db Fa HuB219 LEP R LEP-R LEPR LEPR_HUMAN LEPRD Leptin receptor Leptin receptor fatty Leptin receptor gene related protein Leptin receptor precursor Leptin receptor precursor OB R gene related protein OB receptor OB-R OB-RGRP obl Obr |
Images
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Fig1:
Sandwich ELISA analysis of human Leptin Receptor matched pair antibodies Capture: HA725058, Human Leptin Receptor Rabbit mAb [PSH14-71] Detector: HA725059, Human Leptin Receptor Rabbit mAb [PSH14-72] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725058) diluted in carbonate/bicarbonate buffer, at a concentration of5µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Leptin Receptor protein (HA211148) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725059, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Leptin Receptor in K562 and HepG2 extract samples based on a 1,000 µg/ml extract load. Capture: HA725058, Human Leptin Receptor Rabbit mAb [PSH14-71] Detector: HA725059, Human Leptin Receptor Rabbit mAb [PSH14-72] The concentrations of Leptin Receptor were measured in duplicates, interpolated from the Leptin Receptor standard curve and corrected for sample dilution. Undiluted samples are K562 extract 50% and HepG2 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin Receptor concentration was determined to be 3,255 pg/ml in K562 extract and undetectable in HepG2 extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.