Human VCAM-1 Recombinant Rabbit Monoclonal Antibody [PSH12-47]
cat.: HA725065
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-47 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VCAM-1 aa 25-698 (HA210976). |
| Positive control: | Recombinant Human VCAM-1 protein (HA210976). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-46] to Human VCAM-1 antibody (Capture) (HA725064) and Recombinant Human VCAM-1 protein (HA210976) as the standard. The reference range value is 156-10,000 pg/mL. |
| Uniprot #: | SwissProt: P19320 Human |
| Alternative names: | CD106 CD106 Antigen INCAM 100 INCAM-100 L1CAM MGC99561 V-CAM 1 Vascular Cell Adhesion Molecule 1 Vascular cell adhesion protein 1 VCAM 1 VCAM-1 VCAM1 VCAM1_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725064) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VCAM-1 protein (HA210976) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725065, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native VCAM-1 in human samples. Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Interpolated concentration of native VCAM-1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM-1 concentration was determined to be 1,361,999 pg/mL in human serum. There was no detectable signal in SK-OV-3 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.