Human Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH13-31]
cat.: HA725067
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Osteoprotegerin aa 22-401 (HA210977). |
| Positive control: | Recombinant Human Osteoprotegerin protein (HA210977). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-32] to Human Osteoprotegerin antibody (Detector) (HA725068) and Recombinant Human Osteoprotegerin protein (HA210977) as the standard. The reference range value is 62.5-8,000 pg/mL. |
| Uniprot #: | SwissProt: O00300 Human |
| Alternative names: | MGC29565 OCIF OPG Osteoclastogenesis inhibitory factor Osteoprotegerin PDB5 TNF receptor superfamily member 11b TNFRSF 11B TNFRSF11B TR 1 TR1 TR11B_HUMAN Tumor necrosis factor receptor superfamily member 11B |
Images
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Fig1:
Sandwich ELISA analysis of Human Osteoprotegerin matched pair antibodies Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725067) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Osteoprotegerin protein (HA210977) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725068, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Osteoprotegerin in human serum samples. Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteoprotegerin concentration was determined to be 641 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.