Biotin Conjugated Mouse CRP Recombinant Rabbit Monoclonal Antibody [PSH12-49]
cat.: HA725075B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-49 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CRP aa 20-225 (HA211020). |
| Positive control: | Recombinant Mouse CRP protein (HA211020). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-48] to Mouse CRP antibody (Capture) (HA725073) and Recombinant Mouse CRP protein (HA211020) as the standard. The reference range value is 156-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P14847 Mouse |
| Alternative names: | Pentraxin 1, short C reactive protein C reactive protein pentraxin related C-reactive protein(1-205) CRP CRP_HUMAN MGC88244 Pentraxin 1 PTX 1 PTX1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CRP matched pair antibodies Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725073) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CRP protein (HA211020). starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA725074, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CRP in mouse samples. Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples were 0.00625% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 92,722,639 pg/mL in mouse serum and 2,262 pg/mL in mouse heart cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.