Biotin Conjugated Human Angiogenin Recombinant Rabbit Monoclonal Antibody [PSH12-65]
cat.: HA725078B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Angiogenin aa 24-147 (HA210986). |
| Positive control: | Recombinant Human Angiogenin protein (HA210986). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-64] to Human Angiogenin antibody (Capture) (HA725076) and Recombinant Human Angiogenin protein (HA210986) as the standard. The reference range value is 23.4-3,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P03950 Human |
| Alternative names: | ALS9 AMYOTROPHIC LATERAL SCLEROSIS ANG 1 ANG ANG I ANG1 ANGI ANGI_HUMAN Angiogenin Angiogenin ribonuclease RNase A family, 5 Epididymis luminal protein 168 HEL168 MGC22466 MGC71966 Ribonuclease 5 Ribonuclease RNase A Family 5 RNase 5 RNASE4 RNASE5 |
Images
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Fig1:
Sandwich ELISA analysis of human Angiogenin matched pair antibodies Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725076) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (HA210986) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA725077, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Angiogenin in HepG2, HT-29 and MCF7 cell culture supernatant. Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 2.5%, HT-29 cell culture supernatant 100% and MCF7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 19,596 pg/ml in HepG2 cell culture supernatant, 151 pg/ml in HT-29 cell culture supernatant and undetectable in MCF7 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.