HRP Conjugated Human Angiogenin Recombinant Rabbit Monoclonal Antibody [PSH12-65]
cat.: HA725078H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Angiogenin aa 24-147 (HA210986). |
| Positive control: | Recombinant Human Angiogenin protein (HA210986). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-64] to Human Angiogenin antibody (Capture) (HA725076) and Recombinant Human Angiogenin protein (HA210986) as the standard. The reference range value is 39.1-2,500 pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P03950 Human |
| Alternative names: | ALS9 AMYOTROPHIC LATERAL SCLEROSIS ANG 1 ANG ANG I ANG1 ANGI ANGI_HUMAN Angiogenin Angiogenin ribonuclease RNase A family, 5 Epididymis luminal protein 168 HEL168 MGC22466 MGC71966 Ribonuclease 5 Ribonuclease RNase A Family 5 RNase 5 RNASE4 RNASE5 |
Images
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Fig1:
Sandwich ELISA analysis of human Angiogenin matched pair antibodies Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725076) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (HA210986) starting from 2,500 pg/ml to 0 pg/ml and detect antibody (HA725077, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.