Human ANGPTL3 Recombinant Rabbit Monoclonal Antibody [PSH17-39]
cat.: HA725079
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ANGPTL3 aa 17-460 (HA211029). |
| Positive control: | Recombinant Human ANGPTL3 protein (HA211029). |
| Subcellular location: | Secreted, Cell projection, lamellipodium. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-40] to Human ANGPTL3 antibody (Detector) (HA725080) and Recombinant Human ANGPTL3 protein (HA211029) as the standard. The reference range value is 195.3-25,000 pg/mL. |
| Uniprot #: | SwissProt: Q9Y5C1 Human |
| Alternative names: | ANG 5 ANG-5 ANG5 Angiopoietin 5 Angiopoietin like 3 Angiopoietin related protein 3 Angiopoietin-5 Angiopoietin-like protein 3 Angiopoietin-related protein 3 ANGL3_HUMAN ANGPT5 ANGPTL3 ANL3 FHBL2 OTTHUMP00000010719 UNQ153/PRO179 |
Images
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Fig1:
Sandwich ELISA analysis of Human ANGPTL3 matched pair antibodies Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725079) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ANGPTL3 protein (HA211029) protein starting from 25,000 pg/ml to 0 pg/ml and detect antibody (HA725080, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ANGPTL3 in Human serum. Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Interpolated concentration of native ANGPTL3 was measured in duplicate at different sample concentrations and interpolated from the ANGPTL3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 262,537 pg/ml in Human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.