Human BCMA Recombinant Rabbit Monoclonal Antibody [PSH13-19]
cat.: HA725083
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BCMA aa 1-54 (HA210968). |
| Positive control: | Recombinant Human BCMA protein (HA210968). |
| Subcellular location: | Cell membrane, Endomembrane system. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-18] to Human BCMA antibody (Capture) (HA725082) and recombinant Human BCMA protein (HA210968) as the standard. The reference range value is 31.2-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q02223 Human |
| Alternative names: | B cell maturation antigen B cell maturation factor B cell maturation protein B-cell maturation protein BCM BCMA CD269 CD269 antigen TNFRSF17 TNR17_HUMAN Tumor necrosis factor receptor superfamily member 17 |
Images
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Fig1:
Sandwich ELISA analysis of Human BCMA matched pair antibodies Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725082) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human BCMA protein (HA210968) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725083, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native BCMA in human samples. Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Interpolated concentration of native BCMA was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 10,453 pg/mL in RPMI 8226 and 351 pg/ml in human urine. There was no detectable signal in 293T cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.