Human CD147 Recombinant Rabbit Monoclonal Antibody [PSH13-06]
cat.: HA725085
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD147 aa 22-205 (HA210959). |
| Positive control: | Recombinant Human CD147 protein (HA210959). |
| Subcellular location: | Cell membrane, Melanosome. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-07] to Human CD147 antibody (Detector) (HA725086) and Recombinant Human CD147 protein (HA210959) as the standard. The reference range value is 31.3-4,000 pg/mL. |
| Uniprot #: | SwissProt: P35613 Human |
| Alternative names: | 5A11 antigen 5F7 BASI_HUMAN Basigin (Ok blood group) Basigin Blood brain barrier HT7 antigen Bsg CD 147 CD147 CD147 antigen Collagenase stimulatory factor EMMPRIN Extracellular matrix metalloproteinase inducer Leukocyte activation antigen M6 M 6 M6 M6 leukocyte activation antigen Neurothelin OK OK blood group OK blood group antigen TCSF Tumor cell derived collagenase stimulatory factor Tumor cell-derived collagenase stimulatory factor |
Images
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Fig1:
Sandwich ELISA analysis of human CD147 matched pair antibodies Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725085) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD147 protein (HA210959) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725086, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD147 in human urine samples. Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] The concentrations of CD147 were measured in duplicates, interpolated from the CD147 standard curve and corrected for sample dilution. Undiluted samples are human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD147 concentration was determined to be 493 pg/ml in human urine. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.