Biotin Conjugated Human YKL-40 Recombinant Rabbit Monoclonal Antibody [PSH13-09]
cat.: HA725090B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH13-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human YKL-40 aa 22-383 (HA211030). |
| Positive control: | Recombinant Human YKL-40 protein (HA211030). |
| Subcellular location: | Secreted, extracellular space, Cytoplasm, perinuclear region, Endoplasmic reticulum. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-08] to Human YKL-40 antibody (Capture) (HA725088) and Recombinant Human YKL-40 protein (HA211030) as the standard. The reference range value is 31.3-4,000 pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P36222 Human |
| Alternative names: | 39 kDa synovial protein ASRT7 Cartilage glycoprotein 39 CGP-39 CGP39 CH3L1_HUMAN CHI3L1 chitinase 3 like 1 (cartilage glycoprotein 39) chitinase 3 like 1 Chitinase 3 like protein 1 precursor chitinase Chitinase-3-like protein 1 Chondrocyte protein YKL40 GP 39 GP-39 GP39 HC gp39 HCGP 3P hCGP-39 HCgp39 YKL 40 YKL-40 YKL40 YYL 40 |
Images
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Fig1:
Sandwich ELISA analysis of Human YKL-40 matched pair antibodies Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725089, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load. Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.