HRP Conjugated Human Clusterin Recombinant Rabbit Monoclonal Antibody [PSH15-56]
cat.: HA725093H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Clusterin aa 23-449 (HA210955). |
| Positive control: | Recombinant Human Clusterin protein (HA210955). |
| Subcellular location: | Nucleus, Cytoplasm, Mitochondrion membrane, cytosol, Microsome, Endoplasmic reticulum, Mitochondrion, perinuclear region, Cytoplasmic vesicle, secretory vesicle, chromaffin granule. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-55] to Human Clusterin antibody (Capture) (HA725091) and Recombinant Human Clusterin protein (HA210955) as the standard. The reference range value is 1.370-1000 ng/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10909 Human |
| Alternative names: | 40 AAG 4 AAG4 Aging associated protein 4 Aging-associated gene 4 protein AI893575 APO J Apo-J APOJ ApoJalpha ApoJbeta Apolipoprotein J ApolipoproteinJ CLI CLU CLU1 CLU2 CLUS_HUMAN Clusterin alpha chain Clusterin Clusterin beta chain Complement associated protein SP 40 40 Complement associated protein SP 40 Complement associated protein SP40 Complement cytolysis inhibitor a chain Complement cytolysis inhibitor Complement cytolysis inhibitor b chain Complement lysis inhibitor Complement-associated protein SP-40 D14Ucla3 Dimeric acid glycoprotein Glycoprotein 80 Glycoprotein III GP80 Ku70-binding protein 1 KUB 1 KUB1 MGC24903 NA1/NA2 RATTRPM2B SGP 2 SGP2 SP 40 SP40 Sugp-2 Sulfated glycoprotein 2 Testosterone repressed prostate message 2 Testosterone-repressed prostate message 2 TRPM 2 TRPM-2 TRPM2 TRPM2B Trpmb |
Images
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Fig1:
Sandwich ELISA analysis of Human Clusterin matched pair antibodies Capture: HA725091, Human Clusterin Rabbit mAb [PSH15-55] Detector: HA725092, Human Clusterin Rabbit mAb [PSH15-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725091) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Clusterin protein (HA210955) starting from 1000,000 pg/ml to 0 pg/ml and detect antibody (HA725092, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.