Human FGF-19 Recombinant Rabbit Monoclonal Antibody [PSH13-30]
cat.: HA725101
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FGF-19 aa 25-216 (HA210981). |
| Positive control: | Recombinant Human FGF-19 protein (HA210981). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-29] to Human FGF-19 antibody (Capture) (HA725100) and recombinant Human FGF-19 protein (HA210981) as the standard. The reference range value is 31.2-2,000 pg/mL. |
| Uniprot #: | SwissProt: O95750 Human |
| Alternative names: | FGF 19 FGF-19 FGF15 FGF19 FGF19_HUMAN Fibroblast growth factor 15 Fibroblast growth factor 19 |
Images
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Fig1:
Sandwich ELISA analysis of Human FGF-19 matched pair antibodies Capture: HA725100, Human FGF-19 Rabbit mAb [PSH13-29] Detector: HA725101, Human FGF-19 Rabbit mAb [PSH13-30] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725100) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human FGF-19 protein (HA210981) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725101, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native FGF-19 in human cell culture supernatant samples. Capture: HA725100, Human FGF-19 Rabbit mAb [PSH13-29] Detector: HA725101, Human FGF-19 Rabbit mAb [PSH13-30] Interpolated concentration of native FGF-19 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FGF-19 concentration was determined to be 4,425 pg/mL in HT-29 cell supernatant. There was no detectable signal in Hela cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.