Biotin Conjugated Human Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [PSH13-68]
cat.: HA725111B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH13-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Lipocalin-2 aa 21-198 (HA211028). |
| Positive control: | Recombinant Human Lipocalin-2 protein (HA211028). |
| Subcellular location: | Secreted, Cytoplasmic granule lumen, Cytoplasmic vesicle lumen. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-67] to Human Lipocalin-2 antibody (Capture) (HA725109) and recombinant Human Lipocalin-2 protein (HA211028) as the standard. The reference range value is 46.9-6,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P80188 Human |
| Alternative names: | 24p3 25 kDa alpha 2 microglobulin related subunit of MMP9 25 kDa alpha-2-microglobulin-related subunit of MMP-9 Alpha 2 microglobulin related protein HGNC:6526 HNL Lcn 2 Lcn2 Lipocalin-2 Migration stimulating factor inhibitor MSFI Neutrophil gelatinase associated lipocalin Neutrophil gelatinase associated lipocalin precursor Neutrophil gelatinase-associated lipocalin NGAL NGAL_HUMAN Oncogene 24p3 Oncogenic lipocalin 24p3 p25 Siderocalin siderocalin LCN2 SV40 induced 24P3 protein Uterocalin |
Images
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Fig1:
Sandwich ELISA analysis of Human Lipocalin-2 matched pair antibodies Capture: HA725109, Human Lipocalin-2 Rabbit mAb [PSH13-67] Detector: HA725110, Human Lipocalin-2 Rabbit mAb [PSH13-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725109) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipocalin-2 protein (HA211028) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA725110, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Lipocalin-2 in human samples. Capture: HA725109, Human Lipocalin-2 Rabbit mAb [PSH13-67] Detector: HA725110, Human Lipocalin-2 Rabbit mAb [PSH13-68] Interpolated concentration of native Lipocalin-2 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 269,116 pg/mL in A431 cell culture supernatant and 2,421pg/mL in Human urine. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.