HRP Conjugated Human Neuropilin-1 Recombinant Rabbit Monoclonal Antibody [PSH14-74]
cat.: HA725114H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH14-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Neuropilin-1 aa 22-644 (HA211017). |
| Positive control: | Recombinant Human Neuropilin-1 protein (HA211017). |
| Subcellular location: | Mitochondrion membrane, Cell membrane, Cytoplasm; Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-73] to Human Neuropilin-1 antibody (Capture) (HA725112) and Recombinant Human Neuropilin-1 protein (HA211017) as the standard. The reference range value is 976.6-125,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O14786 Human |
| Alternative names: | A5 protein BDCA4 BLOOD DENDRITIC CELL ANTIGEN 4 CD304 Neuropilin-1 Neuropilin1 NP1 NPN1 NRP 1 NRP NRP1 NRP1_HUMAN transmembrane receptor Vascular endothelial cell growth factor 165 receptor VEGF165R |
Images
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Fig1:
Sandwich ELISA analysis of Human Neuropilin-1 matched pair antibodies Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725112) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropilin-1 protein (HA211017) starting from 125,000 pg/ml to 0 pg/ml and detect antibody (HA725113, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Neuropilin-1 in HT-29 and SK-BR-3 extract samples based on a 1,000 µg/ml extract load. Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73] Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74] The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 extract 50% and SK-BR-3 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 7,020 pg/ml in HT-29 extract and undetectable in SK-BR-3 extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.