Biotin Conjugated Human Thrombopoietin Recombinant Rabbit Monoclonal Antibody [PSH13-97]
cat.: HA725120B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH13-97 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Thrombopoietin aa 22-353 (HA211023). |
| Positive control: | Recombinant Human Thrombopoietin protein (HA211023). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-96] to Human Thrombopoietin antibody (Capture) (HA725118) and recombinant Human Thrombopoietin protein (HA211023) as the standard. The reference range value is 31.3-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P40225 Human |
| Alternative names: | C mpl ligand C-mpl ligand Megakaryocyte colony stimulating factor Megakaryocyte colony-stimulating factor Megakaryocyte growth and development factor Megakaryocyte stimulating factor MGC163194 MGDF MKCSF ML MPL ligand MPLLG Myeloproliferative leukemia virus oncogene ligand Prepro thrombopoietin THCYT1 THPO Thrombopoietin Thrombopoietin nirs variant 1 TPO TPO_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of human Thrombopoietin matched pair antibodies Capture: HA725118, Human Thrombopoietin Rabbit mAb [PSH13-96] Detector: HA725119, Human Thrombopoietin Rabbit mAb [PSH13-97] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725118) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Thrombopoietin protein (HA211023) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725119, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Thrombopoietin in HepG2 cell culture supernatant. Capture: HA725118, Human Thrombopoietin Rabbit mAb [PSH13-96] Detector: HA725119, Human Thrombopoietin Rabbit mAb [PSH13-97] The concentrations of Thrombopoietin were measured in duplicate and interpolated from the Thrombopoietin standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombopoietin concentration was determined to be 47.8 pg/ml in HepG2 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.