Biotin Conjugated Human VEGF Receptor 2 Recombinant Rabbit Monoclonal Antibody [PSH12-84]
cat.: HA725123B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VEGF Receptor 2 aa 20-764 (HA211025). |
| Positive control: | Recombinant Human VEGF Receptor 2 protein (HA211025). |
| Subcellular location: | Cell junction, Endoplasmic reticulum, Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-83] to Human VEGF Receptor 2 antibody (Capture) (HA725121) and Recombinant Human VEGF Receptor 2 protein (HA211025) as the standard. The reference range value is 62.5-8,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P35968 Human |
| Alternative names: | CD309 CD309 antigen EC 2.7.10.1 Fetal liver kinase 1 FLK-1 FLK1 FLK1, mouse, homolog of Kdr Kinase insert domain receptor (a type III receptor tyrosine kinase) Kinase insert domain receptor KRD1 Ly73 Protein tyrosine kinase receptor FLK1 Protein-tyrosine kinase receptor flk-1 soluble VEGFR2 Tyrosine kinase growth factor receptor Vascular endothelial growth factor receptor 2 VEGFR 2 VEGFR VEGFR-2 VEGFR2 VGFR2_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human VEGF Receptor 2 matched pair antibodies Capture: HA725121, Human VEGF Receptor 2 Rabbit mAb [PSH12-83] Detector: HA725122, Human VEGF Receptor 2 Rabbit mAb [PSH12-84] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725121) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGF Receptor 2 protein (HA211025) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725122, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.