Human PCSK9 Recombinant Rabbit Monoclonal Antibody [PSH12-85]
cat.: HA725124
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PCSK9 aa 31-692 (HA210991). |
| Positive control: | Recombinant Human PCSK9 protein (HA210991). |
| Subcellular location: | Cytoplasm, Secreted, Endosome, Lysosome, Cell surface, Endoplasmic reticulum, Golgi apparatus. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-86] to Human PCSK9 antibody (Detector) (HA725125) and Recombinant Human PCSK9 protein (HA210991) as the standard. The reference range value is 20.6-5,000 pg/mL. |
| Uniprot #: | SwissProt: Q8NBP7 Human |
| Alternative names: | Convertase subtilisin/kexin type 9 preproprotein FH3 HCHOLA3 Hypercholesterolemia autosomal dominant 3 LDLCQ1 NARC 1 NARC-1 NARC1 Neural apoptosis regulated convertase 1 Neural apoptosis-regulated convertase 1 PC 9 PC9 PCSK 9 PCSK9 PCSK9_HUMAN Proprotein convertase 9 Proprotein convertase PC9 Proprotein convertase subtilisin/kexin type 9 PSEC0052 Subtilisin/kexin like protease PC9 Subtilisin/kexin-like protease PC9 |
Images
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Fig1:
Sandwich ELISA analysis of Human PCSK9 matched pair antibodies Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725124) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human PCSK9 protein (HA210991) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725125, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract. Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 50% and HepG2 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 9,074 pg/ml in HepG2 cell culture supernatant and 46,405 pg/ml in HepG2 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.