Biotin Conjugated Mouse/Rat Prealbumin Recombinant Rabbit Monoclonal Antibody [PSH15-88]
cat.: HA725129B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-88 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Prealbumin aa 21-147 (HA211118). |
| Positive control: | Recombinant Mouse Prealbumin protein (HA211118). |
| Subcellular location: | Secreted, Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-87] to Mouse/Rat Prealbumin antibody (Capture) (HA725128) and Recombinant Mouse Prealbumin protein (HA211118) as the standard. The reference range value is 117.2-15,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P07309 Mouse | P02767 Rat |
| Alternative names: | Amyloid polyneuropathy Amyloidosis I ATTR Carpal tunnel syndrome 1 CTS CTS1 Dysprealbuminemic euthyroidal hyperthyroxinemia Dystransthyretinemic hyperthyroxinemia Epididymis luminal protein 111 HEL111 HsT2651 PALB Prealbumin amyloidosis type I Prealbumin Prealbumin Thyroxine-binding Senile systemic amyloidosis TBPA Thyroxine binding prealbumin Transthyretin TTHY_HUMAN TTR TTR protein |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Prealbumin matched pair antibodies Capture: HA725128, Mouse/Rat Prealbumin Rabbit mAb [PSH15-87] Detector: HA725129, Mouse/Rat Prealbumin Rabbit mAb [PSH15-88] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725128) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Prealbumin protein (HA211118) starting from 15,000 pg/ml to 0 pg/ml and detect antibody (HA725129, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Prealbumin in mouse serum samples. Capture: HA725128, Mouse/Rat Prealbumin Rabbit mAb [PSH15-87] Detector: HA725129, Mouse/Rat Prealbumin Rabbit mAb [PSH15-88] The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.000046%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 5,702 µg/ml in mouse serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.