Mouse Angiotensinogen Recombinant Rabbit Monoclonal Antibody [PSH17-10]
cat.: HA725132
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Angiotensinogen/AGT aa 25-477 (HA211120). |
| Positive control: | Recombinant Mouse Angiotensinogen/AGT protein (HA211120). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-09] to Mouse Angiotensinogen/AGT antibody (Capture) (HA725131) and Recombinant Mouse Angiotensinogen/AGT protein (HA211120) as the standard. The reference range value is 39.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: P11859 Mouse |
| Alternative names: | Aangiotensinogen (serpin peptidase inhibitor clade A member 8) AGT AI265500 Alpha 1 antiproteinase antitrypsin Ang Ang I Ang II Ang III AngII Angiotensin I Angiotensin II Angiotensin III Angiotensin-3 Angiotensinogen (PAT) Angiotensinogen ANGT_HUMAN ANHU ANRT AT-2 AT-II Des-Asp[1]-angiotensin II FLJ92595 FLJ97926 MGC105326 PAT Pre angiotensinogen Serine (or cysteine) proteinase inhibitor Serpin A8 Serpin peptidase inhibitor clade A member 8 SERPINA8 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Angiotensinogen/AGT matched pair antibodies Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725131) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Angiotensinogen/AGT protein (HA211120) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725132, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native AGT in mouse serum samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse serum 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 12.78 µg/ml in mouse serum. |
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Fig3:
Interpolated concentrations of native AGT in mouse liver lysate samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse liver supernatant 0.2mg/ml. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 72.92 ng/ml in mouse liver lysate. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.