Biotin Conjugated Human Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH15-08]
cat.: HA725142B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Complement C3 23-1663 (HA211092). |
| Positive control: | Recombinant Human Complement C3 protein (HA211092). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-07] to Human Complement C3 antibody (Capture) (HA725140) and Recombinant Human Complement C3 protein (HA211092) as the standard. The reference range value is 3,125-400,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01024 Human |
| Alternative names: | Acylation stimulating protein cleavage product AHUS5 ARMD9 ASP C3 and PZP like alpha 2 macroglobulin domain containing protein 1 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 C3 c3 complement C3adesArg CO3_HUMAN Complement C3 alpha chain Complement C3 Complement C3b alpha' chain Complement C3c alpha' chain fragment 1 Complement C3c alpha' chain fragment 2 Complement C3c alpha'' chain fragment 2 Complement C3d fragment Complement C3dg fragment Complement C3f fragment Complement C3g fragment Complement component 3 Complement factor 3 CPAMD1 HEL S 62p omplement C3 beta chain |
Images
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Fig1:
Sandwich ELISA analysis of Complement C3 matched pair antibodies Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725140) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement C3 protein (HA211092) starting from 400,000 pg/ml to 0 pg/ml and detect antibody (HA725141, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Complement C3 in HepG2 cell culture supernatant. Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 6,576 ng/ml in HepG2 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.