Mouse/Rat Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH16-81]
cat.: HA725143
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH16-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Complement C3 aa 25-1663 (HA211093). |
| Positive control: | Recombinant Mouse Complement C3 protein (HA211093). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-82] to Mouse/Rat Complement C3 antibody (Detector) (HA725144) and Recombinant Mouse Complement C3 protein (HA211093) as the standard. The reference range value is 312.5-40,000 pg/mL. |
| Uniprot #: | SwissProt: P01027 Mouse | P01026 Rat |
| Alternative names: | Acylation stimulating protein cleavage product AHUS5 ARMD9 ASP C3 and PZP like alpha 2 macroglobulin domain containing protein 1 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 C3 CO3_HUMAN Complement C3 Complement C3c alpha' chain fragment 2 Complement component 3 Complement factor 3 CPAMD1 HEL S 62p |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Complement C3 matched pair antibodies Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725143) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement C3 protein (HA211093) protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725144, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Complement C3 in mouse serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in triplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.0005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Complement C3 concentration was determined to be 1,269 µg/ml in mouse serum. |
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Fig3:
Interpolated concentrations of native Complement C3 in rat serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are rat serum 0.000313%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 3,772 µg/ml in rat serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.