Mouse Alpha 1 antitrypsin Recombinant Rabbit Monoclonal Antibody [PSH16-20]
cat.: HA725146
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH16-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Alpha 1 antitrypsin aa 25-413 (HA211161). |
| Positive control: | Recombinant Mouse Alpha 1 antitrypsin protein (HA211161). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-21] to Mouse Alpha 1 antitrypsin antibody (Detector) (HA725147) and Recombinant Mouse Alpha 1 antitrypsin protein (HA211161) as the standard. The reference range value is 7.8-1,000 ng/mL. |
| Uniprot #: | SwissProt: P07758 Mouse |
| Alternative names: | A1A A1AT A1AT_HUMAN AAT Alpha 1 antiproteinase Alpha 1 antitrypsin Alpha 1 antitrypsin null Alpha 1 protease inhibitor Alpha-1 protease inhibitor Alpha-1-antiproteinase alpha1 proteinase inhibitor Alpha1AT Dom1 PI PI1 PRO0684 PRO2275 Serine (or cysteine) proteinase inhibitor clade A member 1 Serine protease inhibitor 1-1 Serine protease inhibitor A1a Serpin A1 Serpin A1a Serpin peptidase inhibitor clade A member 1 Serpina1 Short peptide from AAT SPAAT Spi1-1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Alpha 1 antitrypsin matched pair antibodies Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725146) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Alpha 1 antitrypsin protein (HA211161) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA725147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Alpha 1 antitrypsin in mouse serum. Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] The concentrations of Alpha 1 antitrypsin were measured in duplicates, interpolated from the Alpha 1 antitrypsin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha 1 antitrypsin concentration was determined to be 280 ug/ml in mouse serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.