Mouse/Rat Von Willebrand Factor Recombinant Rabbit Monoclonal Antibody [PSH23-16]
cat.: HA725159
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Von Willebrand Factor aa 20-132 (HA211245). |
| Positive control: | Recombinant Mouse Von Willebrand Factor protein (HA211245). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-15] to Mouse/Rat Von Willebrand Factor (Capture) (HA725158) and Recombinant Mouse Von Willebrand Factor protein (HA211245) as the standard. The reference range value is 625-160,000 pg/mL. |
| Uniprot #: | SwissProt: Q8CIZ8 Mouse | Q62935 Rat |
| Alternative names: | Coagulation factor VIII Coagulation factor VIII VWF F8VWF Factor VIII related antigen von Willebrand antigen 2 von Willebrand antigen II Von Willebrand disease Von Willebrand factor precursor VWD vWF |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse Von Willebrand Factor matched pair antibodies Capture: HA725158, Mouse/Rat Von Willebrand Factor Rabbit mAb [PSH23-15] Detector: HA725159, Mouse/Rat Von Willebrand Factor Rabbit mAb [PSH23-16] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725158) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Von Willebrand Factor protein (HA211245) starting from 160,000 pg/ml to 0 pg/ml and detect antibody (HA725159, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Von Willebrand Factor in mouse serum: Capture: HA725158, Mouse/Rat Von Willebrand Factor Rabbit mAb [PSH23-15] Detector: HA725159, Mouse/Rat Von Willebrand Factor Rabbit mAb [PSH23-16] The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curve and corrected for sample dilution. Undiluted samples are mouse serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 259.14 ng/ml in mouse serum. |
|
Fig3:
Interpolated concentrations of native Von Willebrand Factor in rat serum: Capture: HA725158, Mouse/Rat Von Willebrand Factor Rabbit mAb [PSH23-15] Detector: HA725159, Mouse/Rat Von Willebrand Factor Rabbit mAb [PSH23-16] The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curve and corrected for sample dilution. Undiluted samples are rat serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 612.52 ng/ml in rat serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.