Human Complement factor B Recombinant Rabbit Monoclonal Antibody [PSH19-46]
cat.: HA725161
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-46 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Complement factor B aa 26-764 (HA211099). |
| Positive control: | Recombinant Human Complement factor B protein (HA211099). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-47] to Human Complement factor B antibody (Detector) (HA725162) and Recombinant Human Complement factor B protein (HA211099) as the standard. The reference range value is 7.8-4,000 pg/mL. |
| Uniprot #: | SwissProt: P00751 Human |
| Alternative names: | C3/C5 convertase Complement factor B Glycine-rich beta glycoprotein PBF2 Properdin factor B |
Images
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Fig1:
Sandwich ELISA analysis of Human Complement factor B matched pair antibodies Capture: HA725161, Human Complement factor B Rabbit mAb [PSH19-46] Detector: HA725162, Human Complement factor B Rabbit mAb [PSH19-47] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725161) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement factor B protein (HA211099) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725162, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Human Factor B in human plasma samples. Capture: HA725161, Human Complement factor B Rabbit mAb [PSH19-46] Detector: HA725162, Human Complement factor B Rabbit mAb [PSH19-47] The concentrations of Human Factor B were measured in duplicates, interpolated from the Human Factor B standard curve and corrected for sample dilution. Undiluted samples are human plasma 0.00063%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Human Factor B concentration was determined to be 165.6 ug/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.