Human Renin Recombinant Rabbit Monoclonal Antibody [PSH17-00]
cat.: HA725169
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human Renin aa 24-406 (HA211179). |
| Positive control: | Recombinant Human Renin protein (HA211179). |
| Subcellular location: | Secreted, Membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-01] to Human Renin antibody (Detector) (HA725170) and Recombinant Human Renin protein (HA211179) as the standard. The reference range value is 31.3-4,000 pg/mL. |
| Uniprot #: | SwissProt: P00797 Human |
| Alternative names: | Angiotensin forming enzyme Angiotensin forming enzyme precursor Angiotensinogenase Angiotensinogenase precursor FLJ10761 HNFJ2 REN Ren1 RENI_HUMAN Renin Renin precursor renal |
Images
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Fig1:
Sandwich ELISA analysis of Human Renin matched pair antibodies Capture: HA725169, Human Renin Rabbit mAb [PSH17-00] Detector: HA725170, Human Renin Rabbit mAb [PSH17-01] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725169) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Renin protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725170, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Renin in human serum samples. Capture: HA725169, Human Renin Rabbit mAb [PSH17-00] Detector: HA725170, Human Renin Rabbit mAb [PSH17-01] The concentrations of Renin were measured in duplicates, interpolated from the Renin standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Renin concentration was determined to be 1,544 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.