Human BACE1 Recombinant Rabbit Monoclonal Antibody [PSH16-22]
cat.: HA725171
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH16-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BACE1 aa 22-457 (HA211177). |
| Positive control: | Recombinant Human BACE1 protein (HA211177). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-23] to Human BACE1 antibody (Detector) (HA725172) and Recombinant Human BACE1 protein (HA211177) as the standard. The reference range value is 156-20,000 pg/mL. |
| Uniprot #: | SwissProt: P56817 Human |
| Alternative names: | APP beta secretase Asp 2 ASP2 Aspartyl protease 2 BACE 1 BACE BACE1 BACE1_HUMAN Beta secretase 1 Beta secretase Beta site amyloid beta A4 precursor protein cleaving enzyme Beta site amyloid precursor protein cleaving enzyme 1 Beta site amyloid precursor protein cleaving enzyme Beta site APP cleaving enzyme 1 Beta site APP cleaving enzyme Beta-secretase 1 Beta-site amyloid precursor protein cleaving enzyme 1 Beta-site APP cleaving enzyme 1 FLJ90568 HSPC104 Memapsin 2 Memapsin-2 Memapsin2 Membrane associated aspartic protease 2 Membrane-associated aspartic protease 2 Transmembrane aspartic proteinase Asp2 |
Images
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Fig1:
Sandwich ELISA analysis of Human BACE1 matched pair antibodies Capture: HA725171, Human BACE1 Rabbit mAb [PSH16-22] Detector: HA725172, Human BACE1 Rabbit mAb [PSH16-23] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725171) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human BACE1 protein (HA211177) protein starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725172, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native BACE1 in SH-SY5Y samples based on a 1,000µg/mL extract load. Capture: HA725171, Human BACE1 Rabbit mAb [PSH16-22] Detector: HA725172, Human BACE1 Rabbit mAb [PSH16-23] The concentrations of BACE1 were measured in duplicates, interpolated from the BACE1 standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human BACE1 concentration was determined to be 7417.89 pg/ml in SH-SY5Y extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.