Biotin Conjugated H-FABP Recombinant Rabbit Monoclonal Antibody [PSH16-99]
cat.: HA725178B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human H-FABP aa 1-133 (HA211159). |
| Positive control: | Recombinant Human H-FABP protein (HA211159). |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-98] to Human H-FABP antibody (Capture) (HA725177) and Recombinant Human H-FABP protein (HA211159) as the standard. The reference range value is 15.6-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P05413 Human | P11404 Mouse |
| Alternative names: | 422 protein Cardiac FABP Cardiac Fatty Acid Binding Protein FABP 11 FABP 3 FABP11 FABP3 FABPH_HUMAN Fatty acid binding protein 11 Fatty acid binding protein 3 Fatty acid binding protein 3 muscle and heart Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor Fatty acid binding protein 3 muscle Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) Fatty acid binding protein 3, muscle Fatty acid binding protein heart Fatty acid binding protein, heart Fatty acid binding protein, muscle and heart Fatty acid binding protein, skeletal muscle Fatty acid-binding protein 3 Fatty acid-binding protein H FABP H-FABP heart Heart type fatty acid binding protein Heart-type fatty acid-binding protein M FABP M-FABP Mammary derived growth inhibitor Mammary-derived growth inhibitor MDGI Muscle fatty acid binding protein Muscle fatty acid-binding protein Mylein protein P2 homolog O FABP...... |
Images
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Fig1:
Sandwich ELISA analysis of Human H-FABP matched pair antibodies Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725177) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human H-FABP protein (HA211159) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725178, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native H-FABP in human serum and THP-1 cell supernatant. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and THP-1 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 2,876 pg/ml in human serum and undetectable in THP-1 cell culture supernatant. |
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Fig3:
Interpolated concentrations of native H-FABP in mouse heart extract samples based on a 1,000 μg/ml extract load. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are mouse heart extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 316 pg/ml in mouse heart extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.